ARG63065

anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade)

anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade) for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

Mouse Inflammatory Cell Marker antibody; Neurophil Marker antibody; Mouse MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description Functional grade and low endotoxin Rat Monoclonal antibody [RB6-8C5] recognizes Ly6G + Ly6C
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IHC-P, IP, WB
Specificity This antibody recognizes the mouse Gr-1 antigen, a ~21 - 25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007).
Host Rat
Clonality Monoclonal
Clone RB6-8C5
Isotype IgG2b
Target Name Ly6G + Ly6C
Antigen Species Mouse
Immunogen Murine granulocytes
Conjugation Un-conjugated
Alternate Names Gr1; Gr-1; Ly-6G

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IP1 - 2 µg / 100 - 500 µg of protein
WB1 µg/ml
Application Note Functional studies: This clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (PMID: 8120393, PMID: 17884993).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 546644 Mouse Ly6g

Gene Symbol Ly6g
Gene Full Name lymphocyte antigen 6 complex, locus G
Background Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. _x000D_
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Research Area Mouse Inflammatory Cell Marker antibody; Neurophil Marker antibody; Mouse MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 12 kDa

Images (2) Click the Picture to Zoom In

  • ARG63065 anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade) FACS image

    Flow Cytometry: Murine splenocyte suspension stained with ARG63065 anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade) at 9 µg/ml dilution, followed by PE-conjugated Donkey anti-Rat antibody.

  • ARG63065 anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade) FACS image

    Flow Cytometry: Separation of murine Ly6G positive cells (red-filled) from Ly6G negative cells (black-dashed). Murine splenocyte suspension stained with ARG63065 anti-Ly6G + Ly6C antibody [RB6-8C5] (Functional Grade) at 9 µg/ml dilution, followed by PE-conjugated Donkey anti-Rat antibody.

Clone References

Hemozoin induces hepatic inflammation in mice and is differentially associated with liver pathology depending on the Plasmodium strain.

Deroost K et al.
PLoS One.,  (2014)

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The serine protease inhibitor elafin maintains normal growth control by opposing the mitogenic effects of neutrophil elastase.

Caruso JA et al.
Oncogene.,  (2015)

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Toll-like receptor 9-activation during onset of myocardial ischemia does not influence infarct extension.

IHC-P / Mouse

Ohm IK et al.
PLoS One.,  (2014)

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Alterations in neutrophil production and function at an early stage in the high-fructose rat model of metabolic syndrome.

Tagzirt M et al.
Am J Hypertens.,  (2014)

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Statins prevent cervical remodeling, myometrial contractions and preterm labor through a mechanism that involves hemoxygenase-1 and complement inhibition.

Gonzalez JM et al.
Mol Hum Reprod.,  (2014)

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Intestinal epithelial CD98 directly modulates the innate host response to enteric bacterial pathogens.

ICC/IF / Mouse

Charania MA et al.
Infect Immun.,  (2013)

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Density of Gr1-positive myeloid precursor cells, p-STAT3 expression and gene expression pattern in canine mammary cancer metastasis.

IHC-P / Dog

Król M et al.
Vet Res Commun.,  (2011)

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Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.

FACS / Mouse

Chen D et al.
J Exp Med.,  (2008)

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