ARG65280
anti-LY6E antibody
anti-LY6E antibody for Flow cytometry,ICC/IF,Immunohistochemistry and Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LY6E |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, ICC/IF, IHC |
| Specificity | Reported variants represent identical protein: NP_002337.1, NP_001120685.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LY6E |
| Antigen Species | Mouse |
| Immunogen | SCQEKDHYCIT |
| Conjugation | Un-conjugated |
| Alternate Names | RIGE; Lymphocyte antigen 6E; RIG-E; Retinoic acid-induced gene E protein; TSA-1; Ly-6E; Thymic shared antigen 1; SCA-2; SCA2; Stem cell antigen 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ly6e |
| Gene Full Name | lymphocyte antigen 6 complex, locus E |
| Function | Involved in T-cell development. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 14 kDa |
Images (3) Click the Picture to Zoom In
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ARG65280 anti-LY6E antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed NIH/3T3 cells permeabilized with 0.15% Triton. Cells were stained with ARG65280 anti-LY6E antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65280 anti-LY6E antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed NIH/3T3 cells permeabilized with 0.5% Triton. Cells were stained with ARG65280 anti-LY6E antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG65280 anti-LY6E antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed L929 cells permeabilized with 0.15% Triton. Cells were stained with ARG65280 anti-LY6E antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
