ARG63581
anti-LIME1 antibody
anti-LIME1 antibody for Western blot and Mouse
Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LIME1 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LIME1 |
| Antigen Species | Mouse |
| Immunogen | C-ENVYESIKEMGL |
| Conjugation | Un-conjugated |
| Alternate Names | Lck-interacting molecule; Lck-interacting membrane protein; Lck-interacting transmembrane adapter 1; dJ583P15.4; LIME |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9EQR5 Mouse Lck-interacting transmembrane adapter 1 |
|---|---|
| Gene Symbol | Lime1 |
| Gene Full Name | Lck interacting transmembrane adaptor 1 |
| Background | This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
| Function | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 31 kDa |
| PTM | Palmitoylation of Cys-28 and Cys-31 is required for raft targeting. Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins. |
Images (1) Click the Picture to Zoom In
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ARG63581 anti-LIME1 antibody WB image
Western blot: Mouse Heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63581 anti-LIME1 antibody at 1 µg/ml dilution.
