ARG54859
anti-LIF antibody
anti-LIF antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LIF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LIF |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 72-101 (Center) of Human LIF. |
| Conjugation | Un-conjugated |
| Alternate Names | LIF; Leukemia inhibitory factor; Emfilermin; CDF; DIA; Differentiation-stimulating factor; MLPLI; HILDA; Melanoma-derived LPL inhibitor; D factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | CEM |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIF |
| Gene Full Name | leukemia inhibitory factor |
| Background | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. [UniProt] |
| Cellular Localization | Secreted. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody |
| Calculated MW | 22 kDa |
Images (3) Click the Picture to Zoom In
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ARG54859 anti-LIF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with ARG54859 anti-LIF antibody.
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ARG54859 anti-LIF antibody WB image
Western blot: 35 µg of CEM cell lysate stained with ARG54859 anti-LIF antibody.
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ARG54859 anti-LIF antibody FACS image
Flow Cytometry: CEM cells stained with ARG54859 anti-LIF antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
