ARG44401

anti-LGI4 antibody

anti-LGI4 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LGI4
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name LGI4
Antigen Species Human
Immunogen Human LGI4 recombinant protein (aa. sequence: E225-H531).
Conjugation Un-conjugated
Alternate Names LGI4; Leucine Rich Repeat LGI Family Member 4; Leucine-Rich Glioma-Inactivated Protein 4; Leucine-Rich Repeat LGI Family Member 4; LGI1-Like Protein 3; LGIL3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 163175 Human LGI4

GeneID: 243914 Mouse LGI4

Swiss-port # Q8K1S1 Mouse Leucine-rich repeat LGI family member 4

Swiss-port # Q8N135 Human Leucine-rich repeat LGI family member 4

Gene Symbol LGI4
Gene Full Name Leucine Rich Repeat LGI Family Member 4
Background Involved in regulation of myelination. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in arthrogryposis multiplex congenita-1 and childhood absence epilepsy.
Function Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation.
Cellular Localization Secreted
Calculated MW 59 kDa
PTM Glycoprotein

Images (7) Click the Picture to Zoom In

  • ARG44401 anti-LGI4 antibody IHC-P image

    Immunohistochemistry: Human bladder urothelial carcinoma stained with ARG44401 anti-LGI4 antibody at 2 μg/mL dilution.

  • ARG44401 anti-LGI4 antibody ICC/IF image

    Immunofluorescence: SiHa stained with ARG44401 anti-LGI4 antibody at 5 μg/mL dilution.

  • ARG44401 anti-LGI4 antibody WB image

    Western blot: HepG2 and Hacat stained with ARG44401 anti-LGI4 antibody at 0.5 μg/mL dilution.

  • ARG44401 anti-LGI4 antibody FACS image

    Flow Cytometry: JK stained with ARG44401 anti-LGI4 antibody at 1 μg/10^6 cells dilution.

  • ARG44401 anti-LGI4 antibody WB image

    Western blot: Rat brain and Rat stomach stained with ARG44401 anti-LGI4 antibody at 0.5 μg/mL dilution.

  • ARG44401 anti-LGI4 antibody WB image

    Western blot: Mouse brain tand Mouse stomach stained with ARG44401 anti-LGI4 antibody at 0.5 μg/mL dilution.

  • ARG44401 anti-LGI4 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG44401 anti-LGI4 antibody at 2 μg/mL dilution.