ARG66141
anti-Klotho antibody (Biotin)
anti-Klotho antibody (Biotin) for ELISA,Western blot and Human
Overview
Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes Klotho |
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Tested Reactivity | Hu |
Tested Application | ELISA, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Klotho |
Antigen Species | Human |
Immunogen | CHO cells derived recombinant Human Klotho. (EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH) |
Conjugation | Biotin |
Alternate Names | Klotho; EC 3.2.1.31 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified by affinity chromatography. |
Buffer | PBS (pH 7.2) |
Concentration | 1 mg/ml |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | KL |
Gene Full Name | klotho |
Background | This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008] |
Function | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. [UniProt] |
Calculated MW | 116 kDa |
PTM | N-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG66141 anti-Klotho antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human Klotho stained with ARG66141 anti-Klotho antibody (Biotin), under non-reducing conditions.
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ARG66141 anti-Klotho antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human Klotho stained with ARG66141 anti-Klotho antibody (Biotin), under reducing conditions.