ARG44509

anti-Intersectin 1 antibody

anti-Intersectin 1 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Intersectin 1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Target Name Intersectin 1
Antigen Species Human
Immunogen Human Intersectin 1 recombinant protein
Conjugation Un-conjugated
Alternate Names ITSN1; Intersectin 1; SH3P17; SH3D1A; ITSN; Human Intersectin-SH3 Domain-Containing Protein SH3P17; Src Homology 3 Domain-Containing Protein; Intersectin 1 (SH3 Domain Protein); SH3 Domain-Containing Protein 1A; Intersectin-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 6453 Human ITSN1

Swiss-port # Q15811 Human Intersectin-1

Gene Symbol ITSN1
Gene Full Name Intersectin 1
Background The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
Function Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery.
Cellular Localization Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Synapse, Synaptosome
Calculated MW 195 kDa
PTM Phosphoprotein

Images (3) Click the Picture to Zoom In

  • ARG44509 anti-Intersectin 1 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44509 anti-Intersectin 1 antibody at 5 μg/mL dilution.

  • ARG44509 anti-Intersectin 1 antibody WB image

    Western blot: Caco-2, SiHa and HeLa stained with ARG44509 anti-Intersectin 1 antibody at 0.5 μg/mL dilution.

  • ARG44509 anti-Intersectin 1 antibody FACS image

    Flow Cytometry: SiHa stained with ARG44509 anti-Intersectin 1 antibody at 1 μg/10^6 cells dilution.