ARG44480

anti-INTS12 antibody

anti-INTS12 antibody for Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes INTS12
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Target Name INTS12
Antigen Species Human
Immunogen Human INTS12 recombinant protein
Conjugation Un-conjugated
Alternate Names INTS12; Integrator Complex Subunit 12; PHD Finger Protein 22; SBBI22; INT12; PHF22; Hypothetical Nuclear Factor SBBI22; Int12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 295448 Rat INTS12

GeneID: 57117 Human INTS12

GeneID: 71793 Mouse INTS12

Gene Symbol INTS12
Gene Full Name Integrator Complex Subunit 12
Background INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2.
Function Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Cellular Localization Nucleus
Calculated MW 49 kDa
PTM Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (4) Click the Picture to Zoom In

  • ARG44480 anti-INTS12 antibody WB image

    Western blot: HepG2, K562, Jurkat and HEL stained with ARG44480 anti-INTS12 antibody at 0.5 μg/mL dilution.

  • ARG44480 anti-INTS12 antibody FACS image

    Flow Cytometry: MCF-7 stained with ARG44480 anti-INTS12 antibody at 1 μg/10^6 cells dilution.

  • ARG44480 anti-INTS12 antibody WB image

    Western blot: PC-12 and NRK stained with ARG44480 anti-INTS12 antibody at 0.5 μg/mL dilution.

  • ARG44480 anti-INTS12 antibody WB image

    Western blot: RAW264.7 and SP2/0 stained with ARG44480 anti-INTS12 antibody at 0.5 μg/mL dilution.