ARG66068

anti-IL3 beta antibody

anti-IL3 beta antibody for ELISA,Neutralizing,Western blot and Rat

Overview

Product Description Goat Polyclonal antibody recognizes IL3 beta
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL3 beta
Antigen Species Rat
Immunogen E. coli derived recombinant Rat IL3 beta.
(MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC)
Conjugation Un-conjugated
Alternate Names MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66069 as a detection antibody
Neut0.68 - 1.03 µg/ml (To yield [ND50] of the biological activity of Rat IL-3β (150 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt]
Calculated MW 17 kDa

Images (3) Click the Picture to Zoom In

  • ARG66068 anti-IL3 beta antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-3β stained with ARG66068 anti-IL3 beta antibody, under non-reducing conditions.

  • ARG66068 anti-IL3 beta antibody standard curve image

    Sandwich ELISA: ARG66068 anti-IL3 beta antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66069 anti-IL3 beta antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66068 anti-IL3 beta antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-3β stained with ARG66068 anti-IL3 beta antibody, under reducing conditions.