ARG66428
anti-IL2 antibody [SQab18130]
anti-IL2 antibody [SQab18130] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SQab18130] recognizes IL2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18130 |
| Isotype | IgG1, kappa |
| Target Name | IL2 |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL2 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL2 |
| Gene Full Name | interleukin 2 |
| Background | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008] |
| Function | Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 18 kDa |
Images (4) Click the Picture to Zoom In
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ARG66428 anti-IL2 antibody [SQab18130] ICC/IF image
Immunofluorescence: MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66428 anti-IL2 antibody [SQab18130] (green) at 1:400 and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66428 anti-IL2 antibody [SQab18130] FACS image
Flow Cytometry: MCF-7 cells were stained with ARG66428 anti-IL2 antibody [SQab18130] at 1 µg/ml (red) and without antibody control (black).
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ARG66428 anti-IL2 antibody [SQab18130] WB image
Western blot: 100 ng of Recombinant IL2 protein and 60 µg of MCF-7 cell lysate stained with ARG66428 anti-IL2 antibody [SQab18130] at 1:2000 dilution.
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ARG66428 anti-IL2 antibody [SQab18130] ELISA image
ELISA: Titration curve of ARG66428 anti-IL2 antibody [SQab18130]. Red: IL2; Black: BSA (negative control).
