ARG40992

anti-Hsp 60 antibody

anti-Hsp 60 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Hsp 60
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Hsp 60
Antigen Species Human
Immunogen Recombinant protein corresponding to A260-Q496 of Human Hsp 60.
Conjugation Un-conjugated
Alternate Names Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15510 Mouse HSPD1

GeneID: 3329 Human HSPD1

GeneID: 63868 Rat HSPD1

Gene Symbol HSPD1
Gene Full Name heat shock 60kDa protein 1 (chaperonin)
Background This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
Cellular Localization Mitochondrion matrix. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
Calculated MW 61 kDa

Images (6) Click the Picture to Zoom In

  • ARG40992 anti-Hsp 60 antibody ICC image

    Immunocytochemistry: SMMC-7721 cells stained with ARG40992 anti-Hsp 60 antibody.

  • ARG40992 anti-Hsp 60 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse intestine tissue stained with ARG40992 anti-Hsp 60 antibody.

  • ARG40992 anti-Hsp 60 antibody WB image

    Western blot: 50 µg of Rat kidney, 50 µg of Mouse kidney, 40 µg of HEPA, 40 µg of NRK, 40 µg of PC-12, 40 µg of SW620, 40 µg of A549, 40 µg of A431 and 40 µg of HeLa whole cell lysates stained with ARG40992 anti-Hsp 60 antibody at 0.5 µg/ml dilution.

  • ARG40992 anti-Hsp 60 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG40992 anti-Hsp 60 antibody (green) at 2 µg/ml, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG40992 anti-Hsp 60 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat intestine tissue stained with ARG40992 anti-Hsp 60 antibody.

  • ARG40992 anti-Hsp 60 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG40992 anti-Hsp 60 antibody.