ARG63020

anti-HLA E antibody [MEM-E/08]

anti-HLA E antibody [MEM-E/08] for Flow cytometry,Immunoprecipitation and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-E/08] recognizes HLA E
Tested Reactivity Hu
Tested Application FACS, IP
Specificity The clone MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
Host Mouse
Clonality Monoclonal
Clone MEM-E/08
Isotype IgG1
Target Name HLA E
Immunogen Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Conjugation Un-conjugated
Alternate Names MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: LCL 721.221 (HLA-E transfected)

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3133 Human HLA-E

Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E

Gene Symbol HLA-E
Gene Full Name major histocompatibility complex, class I, E
Background HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt]
Research Area Immune System antibody
Calculated MW 40 kDa

Images (1) Click the Picture to Zoom In

  • ARG63020 anti-HLA E antibody [MEM-E/08] FACS image

    Flow Cytometry: Separation of unstained (black) and stained (red) HLA-E transfected cells. Cells were stained with ARG63020 anti-HLA E antibody [MEM-E/08] at 0.33 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.

ELISA / Human

Ravindranath MH et al.
Mol Immunol.,  (2010)

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