ARG42193
anti-HLA A antibody
anti-HLA A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HLA A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HLA A |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 82-106 of Human HLA A. (EYWDGETRKVKAHSQTHRVDLGTLR) |
| Conjugation | Un-conjugated |
| Alternate Names | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | U87 and HepG2 | ||||||||
| Observed Size | ~ 41 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain |
|---|---|
| Gene Symbol | HLA-A |
| Gene Full Name | major histocompatibility complex, class I, A |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 41 kDa |
| PTM | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG42193 anti-HLA A antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG42193 anti-HLA A antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42193 anti-HLA A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG42193 anti-HLA A antibody.
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ARG42193 anti-HLA A antibody WB image
Western blot: 40 µg of U87 and HepG2 whole cell lysates stained with ARG42193 anti-HLA A antibody at 0.5 µg/ml dilution.
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ARG42193 anti-HLA A antibody WB image
Western blot: 50 µg of sample under reducing conditions. U-87MG and HL-60 whole cell lysates stained with ARG42193 anti-HLA A antibody at 0.5 µg/ml dilution, overnight at 4°C.
