ARG44498

anti-HDGFL2 antibody

anti-HDGFL2 antibody for ICC/IF,Western blot,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes HDGFL2
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Target Name HDGFL2
Antigen Species Human
Immunogen Human HDGFL2 recombinant protein
Conjugation Un-conjugated
Alternate Names HDGFL2; HDGF Like 2; HRP2; Hdgfrp2; Hepatoma-Derived Growth Factor-Related Protein 2; HDGF-Related Protein 2; HDGF-2; HDGF2; HRP-2; Hepatoma-Derived Growth Factor Related Protein 2; Hepatoma-Derived Growth Factor 2; HDGFRP2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.1-0.25 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 84717 Human HDGFRP2

Swiss-port # Q7Z4V5 Human Hepatoma-derived growth factor-related protein 2

Gene Symbol HDGFL2
Gene Full Name HDGF Like 2
Background This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants.
Function Acts as an epigenetic regulator of myogenesis in cooperation with DPF3a (isoform 2 of DPF3/BAF45C).
Cellular Localization Cytoplasm, Nucleus
Calculated MW 74 kDa
PTM Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (3) Click the Picture to Zoom In

  • ARG44498 anti-HDGFL2 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44498 anti-HDGFL2 antibody at 5 μg/mL dilution.

  • ARG44498 anti-HDGFL2 antibody WB image

    Western blot: PC-3, SH-SY5Y and HepG2 stained with ARG44498 anti-HDGFL2 antibody at 0.5 μg/mL dilution.

  • ARG44498 anti-HDGFL2 antibody FACS image

    Flow Cytometry: PC-3 stained with ARG44498 anti-HDGFL2 antibody at 1 μg/10^6 cells dilution.