ARG41936
anti-Glutaminase antibody
anti-Glutaminase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Glutaminase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody recognizes all three isoforms: KGA, GAM and GAC. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Glutaminase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human Glutaminase. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.5.1.2; AAD20; L-glutamine amidohydrolase; KGA; Glutaminase kidney isoform, mitochondrial; GAM; K-glutaminase; GLS; GAC; GLS1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Jurkat | ||||||||||
| Observed Size | 65 and 71 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O94925 Human Glutaminase kidney isoform, mitochondrial |
|---|---|
| Gene Symbol | GLS |
| Gene Full Name | glutaminase |
| Background | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. [UniProt] |
| Cellular Localization | Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion. [UniProt] |
| Highlight | Related products: Glutaminase antibodies; Glutaminase ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Calculated MW | 73 kDa |
Images (1) Click the Picture to Zoom In
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ARG41936 anti-Glutaminase antibody WB image
Western blot: Jurkat cell lysate stained with ARG41936 anti-Glutaminase antibody at 1:1000 dilution.
