TCA intermediate fumarate promotes mitobiogenesis

arigo is proud to announce that our CARM1 / PRMT4 antibody (ARG53996) was used and published in Cell Metabolism (Impact factor 21.567).

Wang et al., reported that TCA intermediate fumarate promotes ME2 dimerization which not only increases mtDNA but also enables mitochondrial protein production. Moreover, they found that the arginine-methylated ME2 inhibit mitobiogenesis that is of particular interest in highly lethal acute myeloid leukemia (AML) which cells have more mitochondria than normal hematopoietic cells. Their results suggest that ME2-mediated fumarate signaling may be targetable in AML treatment. (Wang et al., (2021) Cell Metab. 33(5):1027-1041.)

arigo's CARM1 / PRMT4 antibody (ARG53996) was used to demonstrate the interaction of CARM1 and ME2. Besides, arigo offers a series of assay kits to facilitate the research of TCA cycle and metabolism.

 

Product	Sample	Cat. No.
Citrate Assay Kit	Serum, plasma, urine, tissue/cell lysate, cell culture supernatant	ARG81388
Aconitase Activity Assay Kit	Serum, tissue homogenate, cell lysate	ARG82129
Isocitrate Assay Kit	Serum, tissue homogenate, cell lysate, food, beverage	ARG82172
Isocitrate Dehydrogenase Assay Kit	Tissue extract, cell lysate, cell culture supernatant	ARG82035
alpha-Ketoglutarate Assay Kit	Serum, plasma, tissue homogenate, cell culture supernatan	ARG82662
Succinate Assay Kit	Biological samples, food, beverage	ARG82193
Fumarase Activity Assay Kit	Serum, plasma, erythrocytes, tissue homogenate, cell culture supernatant	ARG82158
Malate Assay Kit	Food, beverage	ARG82178
Malate Dehydrogenase Activity Assay Kit	Serum, plasma, erythrocytes, tissue homogenate, cell culture supernatant	ARG82179
Oxaloacetate Assay Kit	Serum, plasma, tissue homogenate, cell culture supernatant	ARG82187
Glutaminase Assay Kit	Tissue/cell lysate	ARG82767