ARG82129
Aconitase Activity Assay Kit (Colorimetric)
Aconitase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All
Overview
| Product Description | ARG82129 Aconitase Activity Assay Kit (Colorimetric) can be used to measure Aconitase activity in cell lysate, tissue homogenate, serum. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Aconitase |
| Conjugation Note | Read at 565 nm |
| Sensitivity | 0.5 U/L |
| Detection Range | 0.5 - 100 U/L |
| Sample Type | cell lysate, tissue homogenate, serum |
| Sample Volume | 20 µl |
| Alternate Names | HEL60; Aconitase; EC 4.2.1.3; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRP1; Iron regulatory protein 1; IRE-BP 1; Iron-responsive element-binding protein 1; Citrate hydro-lyase; ACONS; IREB1; IREBP; IREBP1 |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 30 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACO1 |
| Gene Full Name | aconitase 1, soluble |
| Background | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
| Function | Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: Aconitase antibodies; Aconitase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
Images (1) Click the Picture to Zoom In
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ARG82129 Aconitase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 50 U/L Aconitase reaction, using ARG82129 Aconitase Activity Assay Kit (Colorimetric).
| Title | Download Link |
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| ARG82129 Aconitase Activity Assay Kit (Colorimetric) User's manual |
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