ARG82158
Fumarase Activity Assay Kit (Colorimetric)
Fumarase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All
Publication1
Overview
| Product Description | ARG82158 Fumarase Activity Assay Kit (Colorimetric) can be used to measure Fumarase activity in serum, plasma, erythrocytes, tissue and cell culture supernatants. |
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| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Fumarase |
| Conjugation Note | Read at 565 nm |
| Sensitivity | 0.4 U/L |
| Detection Range | 0.4 - 70 U/L |
| Sample Type | serum, plasma, erythrocytes, tissue and cell culture supernatants |
| Sample Volume | 20 µl |
| Alternate Names | MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 40 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FH |
| Gene Full Name | fumarate hydratase |
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| Function | Also acts as a tumor suppressor. [UniProt] |
| Cellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt] |
| Highlight | Related products: Fumarase antibodies; Fumarase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
Images (1) Click the Picture to Zoom In
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ARG82158 Fumarase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 70 U/L Fumarase reaction, using ARG82158 Fumarase Activity Assay Kit (Colorimetric).
Specific References
Resolvin D1 reduces mitochondrial damage to photoreceptors of primary retinal cells exposed to high glucose.
FuncSt / Mouse
| Title | Download Link |
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| ARG82158 Fumarase Activity Assay Kit (Colorimetric) User's manual |
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