ARG82035
Isocitrate Dehydrogenase Assay Kit
Isocitrate Dehydrogenase Assay Kit for Functional study and All species
Overview
| Product Description | ARG82035 Isocitrate Dehydrogenase Assay Kit is a detection kit for the quantification of Isocitrate Dehydrogenase Activity in tissue extracts, cell lysate and cell culture supernatants. |
|---|---|
| Tested Reactivity | All |
| Tested Application | FuncSt |
| Target Name | Isocitrate Dehydrogenase |
| Conjugation Note | Read at 410 nm. |
| Sensitivity | 0.2 mmol/l |
| Detection Range | 0.2 - 20 mmol/l |
| Sample Type | Tissue extracts, cell lysate and cell culture supernatants. |
| Sample Volume | 10 µl |
| Alternate Names | IDPC; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; HEL-S-26; HEL-216; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; PICD; IDCD; NADP; Oxalosuccinate decarboxylase |
Application Instructions
| Assay Time | 40 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IDH1 |
|---|---|
| Gene Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| Cellular Localization | Cytoplasm. Peroxisome. [UniProt] |
| Highlight | Related products: Isocitrate Dehydrogenase antibodies; Isocitrate Dehydrogenase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| PTM | Acetylation at Lys-374 dramatically reduces catalytic activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82035 Isocitrate Dehydrogenase Assay Kit typical data demonstration image
ARG82035 Isocitrate Dehydrogenase Assay Kit results of a typical data with optical density reading at 410 nm.
| Title | Download Link |
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| ARG82035 Isocitrate Dehydrogenase Assay Kit User's manual |
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