ARG58865

anti-GPLD1 antibody

anti-GPLD1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GPLD1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GPLD1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 24-160 of Human GPLD1 (NP_001494.2).
Conjugation Un-conjugated
Alternate Names GPI-PLD; Phosphatidylinositol-glycan-specific phospholipase D; GPIPLDM; Glycosyl-phosphatidylinositol-specific phospholipase D; EC 3.1.4.50; PIGPLD1; PI-G PLD; GPI-specific phospholipase D; Glycoprotein phospholipase D; PIGPLD; PLD; GPIPLD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2822 Human GPLD1

GeneID: 291132 Rat GPLD1

Swiss-port # P80108 Human Phosphatidylinositol-glycan-specific phospholipase D

Swiss-port # Q8R2H5 Rat Phosphatidylinositol-glycan-specific phospholipase D

Gene Symbol GPLD1
Gene Full Name glycosylphosphatidylinositol specific phospholipase D1
Background Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
Function This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 92 kDa

Images (2) Click the Picture to Zoom In

  • ARG58865 anti-GPLD1 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG58865 anti-GPLD1 antibody.

  • ARG58865 anti-GPLD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat heart stained with ARG58865 anti-GPLD1 antibody at 1:100 dilution.