ARG59165
anti-GOLGA5 antibody
anti-GOLGA5 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GOLGA5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GOLGA5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-300 of Human GOLGA5 (NP_005104.3). |
| Conjugation | Un-conjugated |
| Alternate Names | Golgin subfamily A member 5; Protein Ret-II; ret-II; Golgin-84; RET-fused gene 5 protein; GOLIM5; RFG5; Cell proliferation-inducing gene 31 protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | 100 kDa, 115 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GOLGA5 |
| Gene Full Name | golgin A5 |
| Background | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013] |
| Function | Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport. [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type IV membrane protein. Note=Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network. [UniProt] |
| Calculated MW | 83 kDa |
| PTM | Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59165 anti-GOLGA5 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG59165 anti-GOLGA5 antibody at 1:1000 dilution.
