ARG63901
anti-GEF5 antibody
anti-GEF5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GEF5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GEF5 |
| Antigen Species | Human |
| Immunogen | C-DKEIDQNSQQEE |
| Conjugation | Un-conjugated |
| Alternate Names | P60; TIM1; Oncogene TIM; Rho guanine nucleotide exchange factor 5; TIM; GEF5; Ephexin-3; p60 TIM; Transforming immortalized mammary oncogene; Guanine nucleotide regulatory protein TIM |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12774 Human Rho guanine nucleotide exchange factor 5 |
|---|---|
| Background | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 177 kDa |
| PTM | Activation of SRC induces tyrosine phosphorylation of ARHGEF5. |
Images (2) Click the Picture to Zoom In
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ARG63901 anti-GEF5 antibody WB image
Western Blot: HeLa Lysate (35 µg protein in RIPA buffer) stained with ARG63901 anti-GEF5 antibody at 0.1 µg/ml dilution.
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ARG63901 anti-GEF5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63901 anti-GEF5 antibody at 3.75 µg/ml dilution followed by AP-staining.
