ARG57117
anti-GCLM antibody [2D12]
anti-GCLM antibody [2D12] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2D12] recognizes GCLM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2D12 |
| Isotype | IgG1, kappa |
| Target Name | GCLM |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-274 of Human GCLM |
| Conjugation | Un-conjugated |
| Alternate Names | GCS light chain; Gamma-ECS regulatory subunit; Glutamate--cysteine ligase regulatory subunit; GLCLR; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48507 Human Glutamate--cysteine ligase regulatory subunit |
|---|---|
| Gene Symbol | GCLM |
| Gene Full Name | glutamate-cysteine ligase, modifier subunit |
| Background | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| Calculated MW | 31 kDa |
Images (2) Click the Picture to Zoom In
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ARG57117 anti-GCLM antibody [2D12] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57117 anti-GCLM antibody [2D12] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57117 anti-GCLM antibody [2D12] WB image
Western blot: 40 µg of HeLa cell lysate stained with ARG57117 anti-GCLM antibody [2D12] at 1:3000.
