ARG40697
anti-GABAA Receptor alpha 1 antibody
anti-GABAA Receptor alpha 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABAA Receptor alpha 1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Hm |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAA Receptor alpha 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 440-456 of Human GABAA Receptor alpha 1. (ATYLNREPQLKAPTPHQ) |
| Conjugation | Un-conjugated |
| Alternate Names | A; EJM; EIEE19; EJM5; Gamma-aminobutyric acid receptor subunit alpha-1; ECA4; GABA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14867 Human Gamma-aminobutyric acid receptor subunit alpha-1 Swiss-port # P62813 Rat Gamma-aminobutyric acid receptor subunit alpha-1 |
|---|---|
| Gene Symbol | GABRA1 |
| Gene Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Background | This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel (By similarity). [UniProt] |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. [UniProt] |
| Calculated MW | 52 kDa |
Images (3) Click the Picture to Zoom In
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ARG40697 anti-GABAA Receptor alpha 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG40697 anti-GABAA Receptor alpha 1 antibody.
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ARG40697 anti-GABAA Receptor alpha 1 antibody WB image
Western blot: Rat brain lysates (both lanes) stained with ARG40697 anti-GABAA Receptor alpha 1 antibody.
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ARG40697 anti-GABAA Receptor alpha 1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa and U-87MG whole cell lysates stained with ARG40697 anti-GABAA Receptor alpha 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
