ARG58505
anti-Factor D antibody
anti-Factor D antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Factor D |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Factor D |
| Antigen Species | Rat |
| Immunogen | E. coli-derived Rat Factor D recombinant protein (Position: I26-A263). |
| Conjugation | Un-conjugated |
| Alternate Names | C3 convertase activator; PFD; Properdin factor D; DF; Complement factor D; ADIPSIN; EC 3.4.21.46; ADN; Adipsin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CFD |
| Gene Full Name | complement factor D (adipsin) |
| Background | The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008] |
| Function | Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 27 kDa |
Images (4) Click the Picture to Zoom In
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ARG58505 anti-Factor D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58505 anti-Factor D antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58505 anti-Factor D antibody WB image
Western blot: 50 µg of samples under reducing conditions. RH35 and PC-12 cell lysates stained with ARG58505 anti-Factor D antibody at 0.5 µg/ml, overnight at 4°C.
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ARG58505 anti-Factor D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58505 anti-Factor D antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58505 anti-Factor D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58505 anti-Factor D antibody at 1 µg/ml dilution, overnight at 4°C.
