ARG41858
anti-FOLR2 antibody
anti-FOLR2 antibody for Flow cytometry,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FOLR2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FOLR2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 18-46 of Human FOLR2. |
| Conjugation | Un-conjugated |
| Alternate Names | Folate receptor 2; BETA-HFR; FBP; Folate receptor beta; Folate receptor, fetal/placental; FBP/PL-1; FR-beta; FR-P3; Placental folate-binding protein; FR-BETA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | MDA-MB-453 | ||||||
| Observed Size | ~ 29 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOLR2 |
| Gene Full Name | folate receptor 2 (fetal) |
| Background | The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | N-glycosylated. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41858 anti-FOLR2 antibody WB image
Western blot: 20 µg of MDA-MB-453 cell lysate stained with ARG41858 anti-FOLR2 antibody at 1:1000 dilution.
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ARG41858 anti-FOLR2 antibody FACS image
Flow Cytometry: HL-60 cells stained with ARG41858 anti-FOLR2 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
