ARG64251
anti-FH / Fumarase antibody
anti-FH / Fumarase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FH / Fumarase |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FH / Fumarase |
| Antigen Species | Human |
| Immunogen | C-HPNDHVNKSQSSND |
| Conjugation | Un-conjugated |
| Alternate Names | MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: Fumarase antibodies; Fumarase ELISA Kits; Anti-Goat IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 55 kDa |
Images (3) Click the Picture to Zoom In
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ARG64251 anti-FH / Fumarase antibody WB image
Western blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG64251 anti-FH / Fumarase antibody at 0.01 µg/ml dilution.
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ARG64251 anti-FH / Fumarase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64251 anti-FH / Fumarase antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64251 anti-FH / Fumarase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64251 anti-FH / Fumarase antibody at 3.75 µg/ml dilution followed by AP-staining.
