ARG44663
anti-FH / Fumarase antibody
anti-FH / Fumarase antibody for Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes FH / Fumarase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | FH / Fumarase |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | FH; Fumarate Hydratase; Fumarase; Fumarate Hydratase, Mitochondrial; EC 4.2.1.2; HsFH; Epididymis Secretory Sperm Binding Protein; HLRCC; MCUL1; FMRD; LRCC; MCL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FH |
| Gene Full Name | Fumarate Hydratase |
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable). [UniProt] |
| Cellular Localization | Chromosome, Cytoplasm, Mitochondrion, Nucleus. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Acetylation, Phosphoprotein. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG44663 anti-FH / Fumarase antibody WB image
Western blot: Ramos stained with ARG44663 anti-FH / Fumarase antibody at 1 µg/mL dilution.
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ARG44663 anti-FH / Fumarase antibody IP image
Immunoprecipitation: Ramos lysate immunoprecipitated with 2.5 µg of ARG44663 anti-FH / Fumarase antibody.
