ARG57146
anti-FH / Fumarase antibody [3F6]
anti-FH / Fumarase antibody [3F6] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [3F6] recognizes FH / Fumarase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3F6 |
| Isotype | IgG2a, kappa |
| Target Name | FH / Fumarase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 44-510 of Human FH / Fumarase |
| Conjugation | Un-conjugated |
| Alternate Names | MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FH |
| Gene Full Name | fumarate hydratase |
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| Function | Also acts as a tumor suppressor. [UniProt] |
| Highlight | Related products: Fumarase antibodies; Fumarase ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Calculated MW | 55 kDa |
Images (2) Click the Picture to Zoom In
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ARG57146 anti-FH / Fumarase antibody [3F6] FACS image
Flow Cytometry: HeLa cell line stained with ARG57146 anti-FH / Fumarase antibody [3F6] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57146 anti-FH / Fumarase antibody [3F6] WB image
Western blot: 40 µg of 1) HeLa, 2) LNCap, 3) A549, 4) HepG2, 5) 293T, 6) K562, and 7) Jurkat cell lysates stained with ARG57146 anti-FH / Fumarase antibody [3F6] at 1:1000.
