ARG56646
anti-FGF basic antibody
anti-FGF basic antibody for ELISA,Neutralizing,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FGF basic |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ELISA, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FGF basic |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human FGF basic (154 aa). (AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS) |
| Conjugation | Un-conjugated |
| Alternate Names | FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF2 |
| Gene Full Name | fibroblast growth factor 2 (basic) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| Highlight | Related products: FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 31 kDa |
| PTM | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
Images (3) Click the Picture to Zoom In
-
ARG56646 anti-FGF basic antibody WB image
Western blot: 250 - 0.24 ng of Human FGF-basic stained with ARG56646 anti-FGF basic antibody, under reducing conditions.
-
ARG56646 anti-FGF basic antibody WB image
Western blot: 250 - 0.24 ng of Human FGF-basic stained with ARG56646 anti-FGF basic antibody, under non-reducing conditions.
-
ARG56646 anti-FGF basic antibody standard curve image
Sandwich ELISA: ARG56646 anti-FGF basic antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56756 anti-FGF basic antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
