ARG67235

anti-Enolase 2 antibody [12F]

anti-Enolase 2 antibody [12F] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes Enolase 2
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 12F
Isotype IgG1
Target Name Enolase 2
Conjugation Un-conjugated
Alternate Names ENO2; Enolase 2; NSE; Gamma-Enolase; 2-Phospho-D-Glycerate Hydro-Lyase; Enolase 2 (Gamma, Neuronal); Neuronal Enriched Enolase; Neuron-Specific Enolase; Neural Enolase; EC 4.2.1.11; Epididymis Secretory Protein Li 279; 2-Phospho-D-Glycerate Hydrolyase; Neuron Specific Gamma Enolase; Neurone-Specific Enolase; Neuron Specific Enolase; HEL-S-279

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:500 - 1:1000
WB1:1000 - 1:12000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
Preservative 0.025% ProClin 300
Stabilizer 20% Glycerol
Concentration 1.01 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13807 Mouse ENO2

GeneID: 2026 Human ENO2

GeneID: 24334 Rat ENO2

Gene Symbol ENO2
Gene Full Name Enolase 2
Background This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. [Uniprot]
Cellular Localization Cell membrane, Cytoplasm, Membrane. [Uniprot]
Calculated MW 47 kDa
PTM Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [Uniprot]

Images (3) Click the Picture to Zoom In

  • ARG67235 anti-Enolase 2 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG67235 anti-Enolase 2 antibody at 1:200 dilution.

  • ARG67235 anti-Enolase 2 antibody WB image

    Western blot: Rat Brain stained with ARG67235 anti-Enolase 2 antibody at 1:1000 dilution.

  • ARG67235 anti-Enolase 2 antibody WB image

    Western blot: Mouse Brain stained with ARG67235 anti-Enolase 2 antibody at 1:1000 dilution.