ARG44656
anti-Enolase 1 antibody
anti-Enolase 1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes Enolase 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Enolase 1 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | ENO1; Enolase 1; Alpha-Enolase; ENO1L1; MBP-1; MPB1; PPH; ENO1 Intronic Transcript 1 (Non-Protein Coding); 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; ENO1 Intronic Transcript 1; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; EC 4.2.1.11; ENO1-IT1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1; MYC Promoter-Binding Protein 1; C-Myc Promoter-Binding Protein; Alpha Enolase Like 1; Tau-Crystallin; Enolase-Alpha; HEL-S-17; EC 4.2.1; MBPB1; MPB-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO1 |
| Gene Full Name | Enolase 1 |
| Background | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Stimulates immunoglobulin production. [UniProt] |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane, Nucleus. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG44656 anti-Enolase 1 antibody IHC-P image
Immunohistochemistry: Human Colon stained with ARG44656 anti-Enolase 1 antibody at 2 µg/mL dilution.
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ARG44656 anti-Enolase 1 antibody WB image
Western blot: SH-SY5Y, U-251MG, MCF-7, A-431, HeLa, THP-1, Jurkat stained with ARG44656 anti-Enolase 1 antibody at 1 µg/mL dilution.
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ARG44656 anti-Enolase 1 antibody IP image
Immunoprecipitation: MCF7 lysate immunoprecipitated with 2.5 µg of ARG44656 anti-Enolase 1 antibody.
