ARG63807
anti-ERLIN2 / SPFH2 antibody
anti-ERLIN2 / SPFH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ERLIN2 / SPFH2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise isoform 1 (NP_009106) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERLIN2 / SPFH2 |
| Antigen Species | Human |
| Immunogen | C-EDEPLETATKEN |
| Conjugation | Un-conjugated |
| Alternate Names | C8orf2; SPFH2; Endoplasmic reticulum lipid raft-associated protein 2; Erlin-2; SPG18; SPFH domain-containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; NET32 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
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ARG63807 anti-ERLIN2 / SPFH2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63807 anti-ERLIN2 / SPFH2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63807 anti-ERLIN2 / SPFH2 antibody WB image
Western blot: 30 µg of Human breast lysate (in RIPA buffer) stained with ARG63807 anti-ERLIN2 / SPFH2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
