ARG66468

anti-ERGIC2 antibody

anti-ERGIC2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ERGIC2
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERGIC2
Antigen Species Human
Immunogen Recombinant full length protein of Human ERGIC2.
Conjugation Un-conjugated
Alternate Names CDA14; PTX1; Endoplasmic reticulum-Golgi intermediate compartment protein 2; cd002; Erv41

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51290 Human ERGIC2

GeneID: 67456 Mouse ERGIC2

Swiss-port # Q96RQ1 Human Endoplasmic reticulum-Golgi intermediate compartment protein 2

Swiss-port # Q9CR89 Mouse Endoplasmic reticulum-Golgi intermediate compartment protein 2

Gene Symbol ERGIC2
Gene Full Name ERGIC and golgi 2
Background ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 [PubMed 11445006]).[supplied by OMIM, Aug 2008]
Function Possible role in transport between endoplasmic reticulum and Golgi. [UniProt]
Cellular Localization Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Nucleus. Note=Cycles between the endoplasmic reticulum and the Golgi. According to a report, localizes to the nucleus (PubMed:11445006). Another report shows a partial localization in the nucleus (PubMed:17980171). [UniProt]
Calculated MW 43 kDa

Images (2) Click the Picture to Zoom In

  • ARG66468 anti-ERGIC2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse kidney. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then incubated with ARG66468 anti-ERGIC2 antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ARG66468 anti-ERGIC2 antibody WB image

    Western blot: HepG2, SKOV3 and Mouse liver lysates stained with ARG66468 anti-ERGIC2 antibody.