ARG44251
anti-ERCC1 antibody
anti-ERCC1 antibody for ICC/IF and Human
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ERCC1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | ICC/IF |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERCC1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human ERCC1 (QVDVKDPQQALKE) |
| Conjugation | Un-conjugated |
| Alternate Names | DNA excision repair protein ERCC-1; RAD10; COFS4; UV20 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07992 Human DNA excision repair protein ERCC-1 |
|---|---|
| Gene Symbol | ERCC1 |
| Gene Full Name | excision repair cross-complementation group 1 |
| Background | Isoform 1:Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.Responsible,in conjunction with SLX4,for the first step in the repair of interstrand cross-links (ICL).Participates in the processing of anaphase bridge-generating DNA structures,which consist in incompletely processed DNA lesions arising during S or G2 phase,and can result in cytokinesis failure.Also required for homology-directed repair (HDR) of DNA double-strand breaks,in conjunction with SLX4. |
| Function | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt] |
| Cellular Localization | Isoform 1: Nucleus. Isoform 2: Cytoplasm. Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. |
| Research Area | Cancer antibody; Gene Regulation antibody |
Images (2) Click the Picture to Zoom In
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ARG44251 anti-ERCC1 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG44251 anti-ERCC1 antibody at 10µg/ml dilution.
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ARG44251 anti-ERCC1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG44251 anti-ERCC1 antibody at 10µg/ml dilution.
