ARG63178

anti-ELMO2 antibody

anti-ELMO2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Goat Polyclonal antibody recognizes ELMO2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity NP_877496.1 and NP_573403.1 are varients that encode the same protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ELMO2
Antigen Species Human
Immunogen C-PKEPSSYDFVYHYG
Conjugation Un-conjugated
Alternate Names CED12; Protein ced-12 homolog A; hCed-12A; Engulfment and cell motility protein 2; CED-12; ELMO-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 6 µg/ml
WB1 - 3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 63916 Human ELMO2

Swiss-port # Q96JJ3 Human Engulfment and cell motility protein 2

Background The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 83 kDa

Images (2) Click the Picture to Zoom In

  • ARG63178 anti-ELMO2 antibody WB image

    Western Blot: Human Brain lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63178 anti-ELMO2 antibody at 2 µg/ml dilution.

  • ARG63178 anti-ELMO2 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Prostate. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63178 anti-ELMO2 antibody at 3.8 µg/ml dilution followed by AP-staining.