ARG66174

anti-EFHD1 antibody

anti-EFHD1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes EFHD1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Specificity The antibody detects endogenous EFHD1 proteins.
Host Mouse
Clonality Monoclonal
Target Name EFHD1
Antigen Species Human
Immunogen Synthetic peptide of Human EFHD1.
Conjugation Un-conjugated
Alternate Names EF-hand domain-containing protein D1; Swiprosin-2; EF-hand domain-containing protein 1; MSTP133; MST133; SWS2; PP3051

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:300
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80303 Human EFHD1

GeneID: 98363 Mouse EFHD1

Swiss-port # Q9BUP0 Human EF-hand domain-containing protein D1

Swiss-port # Q9D4J1 Mouse EF-hand domain-containing protein D1

Gene Symbol EFHD1
Gene Full Name EF-hand domain family, member D1
Background EFHD1 is an EF-hand domain-containing protein that displays increased expression during neuronal differentiation (Tominaga and Tomooka, 2002 [PubMed 12270117]).[supplied by OMIM, Mar 2008]
Calculated MW 27 kDa

Images (22) Click the Picture to Zoom In

  • ARG66174 anti-EFHD1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG66174 anti-EFHD1 antibody (green).

    Left: Target. Middle: DAPI. Right: merge image.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus cancer tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat heart tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat spleen tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse testis tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse colon tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG66174 anti-EFHD1 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.

    Negative control was used by secondary antibody only.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse lung tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse lung tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse lung tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

  • ARG66174 anti-EFHD1 antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66174 anti-EFHD1 antibody (red) at 1:200 dilution (4°C, overnight).

    Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.