ARG43754
anti-EEA1 antibody
anti-EEA1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EEA1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EEA1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to Human EEA1. |
| Conjugation | Un-conjugated |
| Alternate Names | Early endosome antigen 1; Zinc finger FYVE domain-containing protein 2; MST105; MSTP105; ZFYVE2; Endosome-associated protein p162 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa, Placenta, Brain, Spleen, A431 | ||||||||
| Observed Size | ~170 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EEA1 |
| Gene Full Name | early endosome antigen 1 |
| Background | Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProt] |
| Cellular Localization | Cytoplasm. Early endosome membrane; Peripheral membrane protein. [UniProt] |
| Calculated MW | 162 kDa |
Images (3) Click the Picture to Zoom In
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ARG43754 anti-EEA1 antibody ICC/IF image
Immunofluorescence: A431 cells were stained with ARG43754 anti-EEA1 antibody antibody (red) at 1:100 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
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ARG43754 anti-EEA1 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and stained with ARG43754 anti-EEA1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43754 anti-EEA1 antibody WB image
Western blot: 1) Rat brain tissue lysates, 2) Human A549 cell lysates, 3) Human 22RV1 cell lysates, 4) Human Colo320 cell lysates, 5) Human placenta tissue lysates, 6) HeLa cell lysates, stained with ARG43754 anti-EEA1 antibody at 1:1000 dilution, overnight at 4°C.
