ARG42043
anti-Dopamine beta Hydroxylase antibody
anti-Dopamine beta Hydroxylase antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dopamine beta Hydroxylase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dopamine beta Hydroxylase |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 27-56 of Human Dopamine beta Hydroxylase. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.14.17.1; DBM; Dopamine beta-monooxygenase; Dopamine beta-hydroxylase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Rat adrenal gland | ||||||
| Observed Size | ~ 75 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DBH |
| Gene Full Name | dopamine beta-hydroxylase (dopamine beta-monooxygenase) |
| Background | The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008] |
| Function | Conversion of dopamine to noradrenaline. [UniProt] |
| Cellular Localization | Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | N-glycosylated. Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42043 anti-Dopamine beta Hydroxylase antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG42043 anti-Dopamine beta Hydroxylase antibody (green) at 1:25 dilution. Dylight® 554 Phalloidin (red) for cytoplasmic actin staining. DAPI (blue) for nuclear staining.
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ARG42043 anti-Dopamine beta Hydroxylase antibody WB image
Western blot: 20 µg of Rat adrenal gland lysate stained with ARG42043 anti-Dopamine beta Hydroxylase antibody at 1:2000 dilution.
