ARG65662

anti-Dengue virus NS1 antibody [SQab1503] (Biotin)

anti-Dengue virus NS1 antibody [SQab1503] (Biotin) for ELISA and Dengue virus

Microbiology and Infectious Disease antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [SQab1503] recognizes Dengue virus NS1
Tested Reactivity DEN
Tested Application ELISA
Specificity This antibody recognizes monomer, dimer, hexamer of DENV1/2/3/4 NS1 protein.
Host Mouse
Clonality Monoclonal
Clone SQab1503
Isotype IgG
Target Name Dengue virus NS1
Antigen Species Virus
Immunogen Recombinant hexamer Dengue virus NS1 protein from drosophila cell
Conjugation Biotin
Alternate Names Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:5000
Application Note Recommend using ARG65660 Dengue virus NS1 antibody [SQab1501] for WB, ICC/IF studies.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.4), 1% BSA, 10% Glycerol and 0.01% Thimerosal.
Preservative 0.01% Thimerosal
Stabilizer 1% BSA and 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol DENV_gp1
Gene Full Name Dengue virus nonstructural protein 1
Background Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic.
Function Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot]
Highlight Related Antibody Duos and Panels:
ARG30252 Dengue virus NS1 ELISA Antibody Duo
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Dengue virus antibodies; Dengue virus ELISA Kits; Dengue virus Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Exploring Antiviral Immune Response
Research Area Microbiology and Infectious Disease antibody

Images (2) Click the Picture to Zoom In

  • ARG65661 anti-Dengue virus NS1 antibody [SQab1502] & ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) ELISA image

    ELISA: E. coli expressed recombinant Dengue virus type 1-4 NS1 protein (major in monomer) detected by ARG65661 anti-Dengue virus NS1 antibody [SQab1502] as capture antibody (2.5 μg/ml), and ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) (0.5 μg/ml) as detection antibody, followed by incubation with streptavidin-HRP.

  • ARG65661 anti-Dengue virus NS1 antibody [SQab1502] & ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) ELISA image

    ELISA: 293 cells expressed dengue virus type 2 NS1 protein (major in hexamer) detected by ARG65661 anti-Dengue virus NS1 antibody [SQab1502] as capture antibody (2.5 μg/ml), and ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) (0.5 μg/ml) as detection antibody, followed by incubation with streptavidin-HRP.