ARG66438

anti-Cytokeratin 5 antibody

anti-Cytokeratin 5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 5
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 5
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 510-590 of Human Cytokeratin 5.
Conjugation Un-conjugated
Alternate Names DDD1; 58 kDa cytokeratin; Keratin, type II cytoskeletal 5; EBS2; Keratin-5; Cytokeratin-5; CK5; KRT5A; K5; CK-5; Type-II keratin Kb5; DDD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 110308 Mouse KRT5

GeneID: 369017 Rat KRT5

GeneID: 3852 Human KRT5

Gene Symbol KRT5
Gene Full Name keratin 5, type II
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Calculated MW 62 kDa

Images (3) Click the Picture to Zoom In

  • ARG66438 anti-Cytokeratin 5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma stained with ARG66438 anti-Cytokeratin 5 antibody. The picture on the right is blocked with the synthetic peptide.

  • ARG66438 anti-Cytokeratin 5 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG66438 anti-Cytokeratin 5 antibody at 1:1000 dilution.

  • ARG66438 anti-Cytokeratin 5 antibody WB image

    Western blot: HepG2 cell lysate stained with ARG66438 anti-Cytokeratin 5 antibody at 1:1000 dilution.