ARG62978
anti-Cytokeratin 19 antibody [BA-17]
anti-Cytokeratin 19 antibody [BA-17] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [BA-17] recognizes Cytokeratin 19 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone BA-17 reacts with cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BA-17 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 19 |
| Immunogen | Mammary organoids. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT19 |
| Gene Full Name | keratin 19, type I |
| Background | Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| Highlight | Related products: Cytokeratin 19 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients Therapeutic strategies against PDAC |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 44 kDa |
Images (2) Click the Picture to Zoom In
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ARG62978 anti-Cytokeratin 19 antibody [BA-17] WB image
Western blot: MCF-7 cell lysate stained with ARG62978 anti-Cytokeratin 19 antibody [BA-17].
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ARG62978 anti-Cytokeratin 19 antibody [BA-17] IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG62978 anti-Cytokeratin 19 antibody [BA-17].
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Review for anti-Cytokeratin 19 antibody [BA-17]
Application：WB
Sample：MCF7 cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Ocular melanoma and mammary mucinous carcinoma in an African lion.
IHC / Human
Trp53 deletion stimulates the formation of metastatic pancreatic tumors.
IHC-P / Human
Cytokeratin-18 is a useful serum biomarker for early determination of response of breast carcinomas to chemotherapy.
Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2.
IHC-Fr / Mouse
Oncostatin M, a cytokine released by activated mononuclear cells, induces epithelial cell-myofibroblast transdifferentiation via Jak/Stat pathway activation.
WB / Human
