ARG42597
anti-CutA antibody
anti-CutA antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes CutA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_001014433.1; NP_057005.1; NP_001014840.1). Reported variants represent identical protein: NP_001014837.1, NP_001014838.1, NP_057005.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CutA |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human CutA. (C-QVTESVSDSIT) (NP_001014433.1; NP_057005.1; NP_001014840.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Acetylcholinesterase-associated protein; Brain acetylcholinesterase putative membrane anchor; Protein CutA; ACHAP; C6orf82 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Kelly | ||||||
| Observed Size | ~ 16 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CUTA |
| Gene Full Name | cutA divalent cation tolerance homolog (E. coli) |
| Function | May form part of a complex of membrane proteins attached to acetylcholinesterase (AChE). [UniProt] |
| Calculated MW | Isoform A: 21 kDa Isoform B: 19 kDa Isoform C: 17 kDa |
| PTM | O-glycosylated. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42597 anti-CutA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42597 anti-CutA antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42597 anti-CutA antibody WB image
Western blot: 35 µg of Kelly cell lysate (in RIPA buffer) stained with ARG42597 anti-CutA antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
