ARG63691
anti-Clik 1 / STK35 antibody
anti-Clik 1 / STK35 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Clik 1 / STK35 |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Clik 1 / STK35 |
| Antigen Species | Human |
| Immunogen | ELETRMDQVTCAA |
| Conjugation | Un-conjugated |
| Alternate Names | STK35L1; CLP-36-interacting kinase 1; CLIK-1; PDLIM1-interacting kinase 1; Serine/threonine-protein kinase 35 L1; EC 2.7.11.1; Serine/threonine-protein kinase 35; CLIK1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80ZW0 Mouse Serine/threonine-protein kinase 35 Swiss-port # Q8TDR2 Human Serine/threonine-protein kinase 35 |
|---|---|
| Background | The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 58 kDa |
| PTM | Autophosphorylated. |
Images (6) Click the Picture to Zoom In
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ARG63691 anti-Clik 1 / STK35 antibody WB image
Western blot: Human testis lysate (35 µg protein in RIPA buffer) stained with ARG63691 anti-Clik 1 / STK35 antibody at 1 µg/ml dilution.
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ARG63691 anti-Clik 1 / STK35 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63691 anti-Clik 1 / STK35 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63691 anti-Clik 1 / STK35 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Testis. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63691 anti-Clik 1 / STK35 antibody at 10 µg/ml dilution followed by HRP-staining.
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ARG63691 anti-Clik 1 / STK35 antibody WB image
Western blot: 35 µg of HEK293 nucleus (A) and Human breast cancer (B) lysates (in RIPA buffer) stained with ARG63691 anti-Clik 1 / STK35 antibody at 2 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
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ARG63691 anti-Clik 1 / STK35 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63691 anti-Clik 1 / STK35 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63691 anti-Clik 1 / STK35 antibody WB image
Western blot: 35 µg of Mouse (A) and Pig (B) testis lysates (in RIPA buffer) stained with ARG63691 anti-Clik 1 / STK35 antibody at 1 µg/ml (A) and 2 µg/ml (B) dilutions and incubated at RT for 1 hour.
