ARG44248
anti-Claudin 1 antibody
anti-Claudin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Claudin 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Claudin 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Claudin 1. |
| Conjugation | Un-conjugated |
| Alternate Names | ILVASC; Senescence-associated epithelial membrane protein; CLD1; SEMP1; Claudin-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLDN1 |
| Gene Full Name | claudin 1 |
| Background | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008] |
| Function | Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions. CLDN1 acts as a coreceptor for HCV entry into hepatic cells. [UniProt] |
| Cellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Note=Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. [UniProt] |
| Calculated MW | 23 kDa |
Images (2) Click the Picture to Zoom In
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ARG44248 anti-Claudin 1 antibody IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG44248 anti-Claudin 1 antibody at 2 μg / mL dilution.
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ARG44248 anti-Claudin 1 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG44248 anti-Claudin 1 antibody at 5 μg / mL dilution.
