ARG63987
anti-ChAT antibody
anti-ChAT antibody for IHC-Frozen sections,Western blot and Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ChAT |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-Fr, WB |
| Specificity | This antibody is NOT expected to cross-react to Human ChAT. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ChAT |
| Antigen Species | Mouse |
| Immunogen | C-KERARGPTKPSNLD |
| Conjugation | Un-conjugated |
| Alternate Names | CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Chat |
| Background | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] |
| Research Area | Neuroscience antibody |
| Calculated MW | 83 kDa |
Images (2) Click the Picture to Zoom In
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ARG63987 anti-ChAT antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Mouse forebrain tissue stained with ARG63987 anti-ChAT antibody at 1:2000 dilution.
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ARG63987 anti-ChAT antibody WB image
Western blot: 35 µg of Mouse brain (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63987 anti-ChAT antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
