ARG56614

anti-CXCL10 / IP10 antibody

anti-CXCL10 / IP10 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CXCL10 / IP10
Tested Reactivity Hu, Ms
Tested Application ELISA, IHC-P, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CXCL10 / IP10
Antigen Species Human
Immunogen E.coli derived Recombinant Human IP-10 (CXCL10).
(VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP)
Conjugation Un-conjugated
Alternate Names gIP-10; INP10; 10 kDa interferon gamma-induced protein; IP-10; C-X-C motif chemokine 10; 1-73; Small-inducible cytokine B10; IFI10; Gamma-IP10; SCYB10; crg-2; C7; mob-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56728 as a detection antibody
IHC-P1.0 µg/ml
Neut5.0 - 8.0 µg/ml (To yield [ND50] of the biological activity of Human IP - 10 (100.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15945 Mouse CXCL10

GeneID: 3627 Human CXCL10

Swiss-port # P02778 Human C-X-C motif chemokine 10

Swiss-port # P17515 Mouse C-X-C motif chemokine 10

Gene Symbol CXCL10
Gene Full Name chemokine (C-X-C motif) ligand 10
Background This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014]
Function Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. [UniProt]
Highlight Related products:
CXCL10 antibodies; CXCL10 ELISA Kits; CXCL10 recombinant proteins; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 11 kDa
PTM CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.

Images (5) Click the Picture to Zoom In

  • ARG56614 anti-CXCL10 / IP10 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human carcinoid tissue. The recommended ARG56614 anti-CXCL10 / IP10 antibody concentration is 1.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56614 anti-CXCL10 / IP10 antibody WB image

    Western blot: 1.5 - 3.0 ng of Human IP-10 stained with ARG56614 anti-CXCL10 / IP10 antibody, under reducing conditions.

  • ARG56614 anti-CXCL10 / IP10 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human carcinoid tissue. The recommended ARG56614 anti-CXCL10 / IP10 antibody concentration is 1.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56614 anti-CXCL10 / IP10 antibody WB image

    Western blot: 1.5 - 3.0 ng of Human IP-10 stained with ARG56614 anti-CXCL10 / IP10 antibody, under non-reducing conditions.

  • ARG56614 anti-CXCL10 / IP10 antibody standard curve image

    Sandwich ELISA: ARG56614 anti-CXCL10 / IP10 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56728 anti-CXCL10 / IP10 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.