ARG58556
anti-CSTF3 / CstF 77 antibody
anti-CSTF3 / CstF 77 antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes CSTF3 / CstF 77 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CSTF3 / CstF 77 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human CSTF3 / CstF 77 (NP_001317.1). (C-RETKGKLPSYKE) |
| Conjugation | Un-conjugated |
| Alternate Names | CF-1 77 kDa subunit; Cleavage stimulation factor subunit 3; CSTF-77; CstF-77; CSTF 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||
| Observed Size | ~ 75 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12996 Human Cleavage stimulation factor subunit 3 |
|---|---|
| Gene Symbol | CSTF3 |
| Gene Full Name | cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa |
| Background | The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. [UniProt] |
| Calculated MW | 83 kDa |
Images (1) Click the Picture to Zoom In
-
ARG58556 anti-CSTF3 / CstF 77 antibody WB image
Western blot: 35 µg of HeLa nuclear lysate (in RIPA buffer) stained with ARG58556 anti-CSTF3 / CstF 77 antibody at 0.1 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
