ARG42094
anti-CRLS1 antibody
anti-CRLS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes CRLS1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_061968.1; NP_001120930.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CRLS1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human CRLS1. (C-RSALGSALDPLADK) (NP_061968.1; NP_001120930.1) |
| Conjugation | Un-conjugated |
| Alternate Names | CLS; CLS1; GCD10; C20orf155; dJ967N21.6; Cardiolipin synthase (CMP-forming); CLS; EC 2.7.8.41; Protein GCD10 homolog |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HEK293 | ||||||
| Observed Size | ~ 33 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UJA2 Human Cardiolipin synthase (CMP-forming) |
|---|---|
| Gene Symbol | CRLS1 |
| Gene Full Name | cardiolipin synthase 1 |
| Background | This gene encodes a member of the CDP-alcohol phosphatidyltransferase class-I family of proteins. The encoded enzyme catalyzes the synthesis of cardiolipin, a phospholipid component of mitochondrial membranes that is critical for mitochondrial function. [provided by RefSeq, Apr 2016] |
| Function | Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 33 kDa |
Images (2) Click the Picture to Zoom In
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ARG42094 anti-CRLS1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42094 anti-CRLS1 antibody at 5 µg/ml dilution.
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ARG42094 anti-CRLS1 antibody WB image
Western blot: 35 µg of HEK293 cell lysate (in RIPA buffer) stained with ARG42094 anti-CRLS1 antibody at 0.2 µg/ml dilution and incubated at RT for 1 hour.
