ARG64341

anti-CRHR1 / CRF-R antibody

anti-CRHR1 / CRF-R antibody for ICC/IF,Western blot and Human,Mouse,Rat

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description

Goat Polyclonal antibody recognizes CRHR1 / CRF-R

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CRHR1 / CRF-R
Antigen Species Human
Immunogen C-NEEKKSKVHYH
Conjugation Un-conjugated
Alternate Names CRH-R-1; CRF-R1; CRFR-1; CRFR1; Corticotropin-releasing factor receptor 1; CRH-R1; Corticotropin-releasing hormone receptor 1; CRF-R-1; Crhr

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay - dependent
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12921 Mouse CRHR1

GeneID: 1394 Human CRHR1

GeneID: 58959 Rat CRHR1

Background This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants, one of which represents a read-through transcript with the neighboring gene MGC57346. [provided by RefSeq, Jan 2012]
Research Area Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 51 kDa

Images (2) Click the Picture to Zoom In

  • ARG64341 anti-CRHR1 / CRF-R antibody WB image

    Western Blot: human colon lysate (35 µg protein in RIPA buffer) stained with ARG64341 anti-CRHR1 / CRF-R (aa 107 - 117) antibody at 0.1 µg/ml dilution.

  • ARG64341 anti-CRHR1 / CRF-R antibody WB image

    Western blot: 35 µg of Human colon (A), Human cerebellum (B), Rat brain (C) and Mouse spinal cord (D) lysates (in RIPA buffer) stained with ARG64341 anti-CRHR1 / CRF-R antibody at 1 µg/ml dilution and incubated at RT for 1 hour.